Table 1- Testing Protocol
Sample # |
Bacterial Injector |
KDF Column |
Description of Purpose | |
---|---|---|---|---|
1 | Off | Off | Control: | Measure survival of Ps. fluorescens in water that has passed through the system KDF off by inoculating after collection. |
2 | Off | On | KDF Elution: |
Measure survival in water that has passed through KDF by inoculating after collection. |
3 | On | Off | Control: | Measure survival of Ps. fluorescens that have been injected into the system with KDF off. |
4 | On | On | KDF Contact: |
Measure survival of Ps. fluorescens that have come in direct contact with KDF. |
All water used for testing was deionized and injected with AOAC synthetic hard water to a final concentration of 100 ppm and a pH 7.0 +/-.2
Following collection, the samples were surface plated on Plate Count Agar within 2 to 3 minutes of collection and again after incubation at room temperature for 3 hours.
The same inoculums of Pseudomonas fluorescens was used for injection in to Samples 3 and 4 or for addition to Samples 1 and 2 (added after collection of the water).